Application

Use undiluted. Refer to Kit Package Insert for directions.

Disclaimer

For in vitro Diagnostic UseCE Mark

General description

VitroCol^® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblasts to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.VitroCol^® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains high monomer content as measured by gel permeation chromatography.VitroCol^® is especially ideal for human cell culture systems when coating of surfaces and providing preparations of thin layers of culturing cells. It is not recommend for the formation of a solid gel. VitroCol^® is supplied as a sterile, lyophilized powder.

Immunogen

~250 tests, F(ab)′2 fragment

Legal Information

VitroCol is a registered trademark of Advanced BioMatrix, Inc.

Physical form

Liquid provided as a solution in Phosphate Buffered Saline (PBS), containing 0.02% Evans Blue counterstain, 0.2% BSA, and 0.1% Sodium Azide as a preservative.

Preparation Note

This product is supplied as a lyophilized powder with 15 mg of human collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Specificity

Secondary detection reagent useful for all non-CMV related Light Diagnostic IFA reagents. CMV reagents require catalog number 5024

Storage and Stability

When stored at 2-8°C and protected from light, this reagent is stable up to the expiration date printed on the label. Do not freeze or expose to elevated temperatures.

Warnings / Precautions:

1) Sodium Azide can form potentially explosive metal azides with plumbing. Flush with large volumes of water to prevent azide build up. 2) Avoid contact with Evans Blue, as it is a potential carcinogen. If skin contact occurs, flush with large volumes of water.