Corning® Endothelial Cell Growth Supplement (ECGS), 15mg, is a broadly used supplement to culture a variety of cells, particularly endothelial cells. ECGS contains various growth factors (e.g., acidic FGF or ECGF-a).

• Source: bovine brain
• Quality: tested for ability to promote proliferation of Fetal Bovine Heart Endothelial Cells (FBHEC) in medium containing 10% newborn calf serum, filtered (0.2µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
• Preparation and storage: stable for at least three months at 2° to 8°C (lyophilized) or = one month at -20°C (solubilised), avoid multiple freeze thaws
• Recommended concentration - 50 - 150µg/mL of medium depending on cell type, some primary cells require supplementation of 1-100µg/mL heparin
• Formulation: lyophilised from NaCl-containing buffer with streptomycin sulfate; reconstitute in serum-free medium
• Storage: shelf life: ≥3 months from date of shipment at 2 - 8°C (lyophilised) or ≥one month at -20°C (solubilised)