Analysis Note

Contaminants: none detected by IEP using anti-fibronectin and anti-rat serum

single major band by SDS-PAGE

General description

Native fibronectin purified from pooled rat plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

Please refer to vial label for lot-specific concentration.

500 µg in Plastic ampoule

Physical form

In 100 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3, preservative and reductant-free.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Be VERY careful during the thawing process. Place the vial in a 37°C water bath and leave it there until it is completely thawed. DO NOT DISTURB OR REMOVE THE VIAL AT ANY TIME DURING THE THAWING PERIOD. If the vial is disturbed or removed prior to complete thawing, the product will gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing.

Warning

Toxicity: Standard Handling (A)