Application

Research CategoryStem Cell Research

Research Sub CategoryMesenchymal Stem Cells

This Anti-Brachyury Antibody, clone 3E4.2 is validated for use in WB for the detection of Brachyury.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine.

Immunogen

Synthetic human brachyury peptide, a.a. 224-435.

Epitope: C-terminus

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Format: Purified

Quality

Routinely evaluated by Western blot on NT2 cell lysates.

Western Blotting:
Recommended working dilutions are 1:1000.

Specificity

Reactivity with other species has not been tested.

This monoclonal antibody recognizes the brachyury protein.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.

Target description

47 kDa