F6636-10X1L Display Image

Nutrient Mixture F-12 Ham, powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, Coons Modification, suitable for cell culture

Code: F6636-10X1L D2-231

Application

Coon's modification of Ham's F-12 was developed for culturing hybrid cells that were produced by viral fusion. The modification consists of doubling the amino aci...


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Application

Coon's modification of Ham's F-12 was developed for culturing hybrid cells that were produced by viral fusion. The modification consists of doubling the amino acids and pyruvate and including ascorbic acid. The salt concentrations have also been altered. The formula contains 0.863 mg/L zinc sulfate, which may render it unsuitable for culturing mouse L-cells.

Quantity

Formulated to contain 11.5 grams of powder per liter of medium.

Reconstitution

Supplement with 2.68 g/L sodium bicarbonate.

componentsphenol red: 0.00125 g/Lglucose: 1.802 g/LL-glutamine: 0.292 g/Lsodium pyruvate: 0.22 g/L
formpowder
shipped inambient
storage temp.2-8°C
technique(s)cell culture | hybridoma: suitable, cell culture | mammalian: suitable
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