Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (human umbilical vein endothelial cells).

Biochem/physiol Actions

VEGF165 belongs to the PDGF/VEGF growth factor family characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF is secreted by the majority of tumor cells and initiates angiogenesis by activating endothelial cells of existing blood vessels and promoting their migration.VEGF has also been implicated in correlation with poor prognosis in breast cancer.In addition, VEGF is released in rheumatoid arthritis in response to TNF-α, increasing endothelial permeability and stimulating angiogenesis (formation of capillaries).

General description

VEGF165 is expressed in human HEK 293 cells as a glycosylated homodimer with an apparent molecular mass of 45 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.