Prostaglandin E2, synthetic, powder, BioReagent, suitable for cell culture

Code: P0409-1MG D2-231

Application

Prostaglandin E2has been used as a α-MEM medium supplement for the co-culture of bone marrow macrophages and differentiated osteoblast. It has also been used in t...


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Your Price
€383.00 1MG
€471.09 inc. VAT

Application

Prostaglandin E2has been used as a α-MEM medium supplement for the co-culture of bone marrow macrophages and differentiated osteoblast. It has also been used in the characterization of cells and in vitro decidualization.

Biochem/physiol Actions

Prostaglandin E2 is a signaling molecule produced by activated platelets. The release of PGE2 by activated platelets is part of a mechanism by which activated platelets utilize adjacent erythrocytes to help in clot formation. This product was shown to lower the filterability of human erythrocytes by approximately 30% at a concentration of 10-10sup M and also caused a reduction in mean cell volume by about 10%. The cause of cell shrinkage was the induction of a PGE2- stimulated K+ efflux pathway leading to rapid loss of cellular K+ ions. This loss was shown to be Ca2+dependent. PGE2 has been shown to stimulate the production of interleukin-6 (IL-6) by neonatal mouse parietal bones. After 6 hours in culture, cells stimulated with 10-8sup M PGE2 produced significantly more IL-6 than controls. The pyrogenic activity of PGE2 was not inhibited by dexamethasone, unlike prostaglandin F2α.Most biologically active prostaglandin. PGE2 induces cervical ripening and parturition; mediates bradykinin-induced vasodilation; regulates adenylyl cyclase. Tumor cells that over-express cyclooxygenase 2 display increased invasiveness, angiogenesis, and resistance to apoptosis that may be due to the PGE2-induced expression of angiogenic factors and stabilization of the anti-apoptotic protein, survivin.

Most biologically active prostaglandin. PGE2 induces cervical ripening and parturition; mediates bradykinin-induced vasodilation; regulates adenylyl cyclase. Tumor cells that over-express cyclooxygenase 2 display increased invasiveness, angiogenesis, and resistance to apoptosis that may be due to the PGE2-induced expression of angiogenic factors and stabilization of the anti-apoptotic protein, survivin.The effect of PGE2 on the immune system is mixed. It inhibits T cell activation in vitro, suggesting it is an immunosuppressant. However, in vivo, it appears to effect expansion of the Th17 subset and differentiation of the Th1 subset of T helper cells, marking it as an immunoactivator.

General description

Prostaglandin E2is a lipid that is obtained from arachidonic acid. Prostaglandin hormone is synthesized by the activity of cycloxygenase-2. Prostaglandin E2controls inflammatory responses and vascular smooth muscle activity.

Packaging

1, 5, 10 mg in glass bottle

Physical form

powder -20 °C; stock-frozen in working aliquots, avoid repeated freeze/thaw

assay≥93% (HPLC)
biological sourcesynthetic
formpowder
Gene Informationhuman ... PTGER1(5731), PTGER2(5732), PTGER3(5733), PTGER4(5734), PTGIR(5739)mouse ... Ptger1(19216), Ptger2(19217), Ptger3(19218), Ptger4(19219)
InChI keyXEYBRNLFEZDVAW-ARSRFYASSA-N
InChI1S/C20H32O5/c1-2-3-6-9-15(21)12-13-17-16(18(22)14-19(17)23)10-7-4-5-8-11-20(24)25/h4,7,12-13,15-17,19,21,23H,2-3,5-6,8-11,14H2,1H3,(H,24,25)/b7-4-,13-12+/t15-,16+,17+,19+/m0/s1
potency0.25-100 ng/mL
product lineBioReagent
Quality Level200
shipped inambient
SMILES stringO[C@@H]1CC([C@H](C/C=CCCCC(O)=O)[C@H]1/C=C/[C@@H](O)CCCCC)=O
solubilityacetone: 10 mg/mL, clear, colorless to faintly yellow
storage temp.−20°C
technique(s)cell culture | mammalian: suitable
Code
Description
Unit Size
List Price
Qty
P0409-5MG
Unit:5MG
List Price: €1,560.00
Source:List Price
ADD
Cas Number363-24-6
Pack1MG
This product has met the following criteria: