Application

N1 Medium Supplement (100×) has been used as:a component of the proliferation medium for culturing oligodendrocyte-like cells and neuroblastoma cellsa supplement in the Dulbecco′s modified Eagle medium (DMEM/F12) to culture human oligodendrocytesa component of the α-minimum essential medium (MEM) for culturing induced primary retinal pigment epithelium (ipRPE) monolayers

General description

N1 Medium Supplement (100×) can be used as a supplement to produce complete N1 neural cell growth media or closely related media.

Other Notes

Contains 0.5mg/ml recombinant human insulin, 0.5mg/ml human transferrin (partially iron-saturated), 0.5µg/ml sodium selenite, 1.6mg/ml putrescine, and 0.73µg/ml progesterone.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.