Analysis Note

The proliferative activity is tested in culture using quiescent NR6R-3T3 fibroblasts.

Application

Fibroblast Growth Factor-Acidic human has been used: as a component of growth factor cocktail for treating neural stem cell (NSC) transplantsas a component of Human Neural Stem Cell(HNSC) proliferation medium for Neural Tissue Spheres (NTS) propagationas a growth factor component in fibrin matrix to support embryonic day 14 (E14) cells graft survival

Biochem/physiol Actions

Fibroblast Growth Factor-Acidic (FGF1) is a potent mitogenic agent associated with a variety of tissue origins including liver, vasculature, and skin. These include for a wide variety of mesoderm-derived cells including BALB/c 3T3 fibroblasts, capillary and endocardial endothelial cells, myoblasts, vascular smooth muscle cells, mesothelial cells, glial and astroglial cells, and adrenal cortex. FGF1 participates in glucose homeostasis, adipose tissue remodeling and is a key transducer of the nuclear receptor, peroxisome proliferator-activated receptor γ (PPARγ). FGF1 is a neuronal cell neurotrophic factor and binds to most of the isoforms of the FGF receptor (FGFR) isoforms. Along with FGF2, FGF1 plays a key role in angiogenesis and favors blood vessel formation in chronic inflammatory diseases and cancer. The FGF1 mutant R50E leads to suppression of its angiogenic potential making it a potential anti-angiogenesis agent.

General description

Fibroblast Growth Factor-Acidic (FGF1) belongs to the FGF family and comprises a nuclear export sequence (NES). FGF1 gene is mapped to human chromosome 5q31.3. FGF1 is highly expressed in the central and peripheral nervous system.

Physical form

The product is lyophilized from a 0.2 µm filtered solution of 20 mM Tris, 1 M NaCl, 5 mM DTT containing 50 µg of bovine serum albumin per 1 µg of cytokine.