Application

Nutrient Mixture F-12 Ham has been used:to culture Chinese hamster ovary cells overexpressing insulin receptor (CHO-IR)to culture LAN-1 cellsto maintain SUM149, SUM159, SUM190, FC-IBC02, BCX010, and IBC3 cells

General description

Ham′s Nutrient Mixtures were mainly created to support the clonal growth of numerous clones of Chinese hamster ovary (CHO) cells and clones of HeLa and mouse L-cells. It is also used to grow primary rat hepatocytes and rat prostate epithelial cells. Ham′s F-12 is used as the medium of choice for a clonal toxicity assay using CHO cells.