Application

Medium 199 has been used: as a suspension media to maintain the primary neuronal cultures as a component of the collagen gel for ex vivo embryonic heart culture to maintain the ovarian tissue sections as part of the slow-freezing protocol

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Complete liquid version of the original M 199 formulation published by Morgan.