Minimum Essential Medium Eagle, Modified, with Earles salts and reduced NaHCO3. without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Application

Recommended for use in a carbon dioxide-free culture system.

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle’;s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks’; or Earle’;s salts has broadened the usefulness of this medium.

Other Notes

May also be supplemented with HEPES to raise the buffering range.

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln.

Reconstitution

Supplement with 0.292 g/L L-glutamine.