Application

Earle′s Balanced Salt Solution 10x has been used:as an additive to the nutritive medium sheep nematode Trichostrongylus colubriformis eggsin the swim-up method to prepare human sperm samplein the steady-state medium to culture bovine primary mammary epithelial cells

Features and Benefits

Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. When combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism. Provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

General description

Earle′s Balanced Salt Solution 10x is a saline solution with physiological pH. It is useful in inducing autophagy and promotes Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) maturation.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate at 1×.