Ham's F-12 media was originally formulated for the serum-free culture of CHO cells and is based Ham's F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids. This medium is also suitable for carcinoma cells, rat skeletal myoblasts, Chinese hamster lung cells, and rat, rabbit, and chicken embryos. This formulation contains L-glutamine and does not contain sodium bicarbonate.
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