TRUEGEL3D POLYMER

Application

FAST-DEXTRAN polymer is used with crosslinkers (CD/PEG crosslinkers) to establish stable hydrogels

Caution

Avoid frequent freeze-thaw cycles and store FAST-DEXTRAN in small portions

Components

TRUEDEXF contains FAST-DEXTRAN lyophilized with reconstitution buffer, TrueGel3D thioglycerol and water

Features and Benefits

Contains dextrose polymer functionalized with maleimide groups for quick gelation Degradable polymer

General description

Dextrose polymers are functionalized with thiol-reactive maleimide groups, which react rapidly with thiol groups of crosslinkers to form hydrogels in few seconds. The thiol-reactive maleimide groups are also compatible to conjugate polymers with bioactive factors. The thioglycerol is used to maintain hydrogel strength and also as a control to compare cellular behavior in peptide-modified versus unmodified hydrogels. The buffers (pH7.2 and pH 5.5) are used to control pH and osmotic conditions to optimize time for gelation.TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.