Application

TRUE6-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic natural tissue stiffness and to investigate the effect of mechanical stimulus on cell physiology and behavior. It is also used to culture non-proliferating cells and proliferating spheroids that do not attach to hydrogel for survival, but rather use their own extracellular matrix.

Caution

Avoid prolong exposure of PEG non cell-degradable crosslinker to air.

Components

TRUE6-1KT contains SLO-DEXTRAN solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer and water.

Features and Benefits

Slow gelling (takes 8 minutes to 25 minutes, depends on gel stiffness) Flexibility in modulating gel stiffness Does not allow cell migration Allow cell recovery and post-culture analyses Cell inert hydrogel (if not customized with RGD peptide)

General description

The gel is formed by stable thioether bonds between thiol groups on PEG non cell-degradable crosslinker and thio-reactive groups on SLO-DEXTRAN. The polymer, SLO-DEXTRAN is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-DEXTRAN. SLO-DEXTRAN does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.