Application
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.
Diethyl pyrocarbonate was used as:nuclease inhibitor for the extraction of undegraded nucleic acids from etiolated and green plant tissuesan antimicrobial additiveribonuclease inhibitor histidine residue modifierreagent for conversion of imines to carbamates
Biochem/physiol Actions
Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.
General description
Diethyl pyrocarbonate reacts selectively with the single-stranded loops of cruciform structures. The irreversible inactivation of ribonuclease with diethylpyrocarbonate has been studied.
Packaging
5 g in glass bottle
25, 100 g in poly bottle
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