Diethyl pyrocarbonate, 97%

Application

Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Diethyl pyrocarbonate was used as:nuclease inhibitor for the extraction of undegraded nucleic acids from etiolated and green plant tissuesan antimicrobial additiveribonuclease inhibitor histidine residue modifierreagent for conversion of imines to carbamates

Biochem/physiol Actions

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

General description

Diethyl pyrocarbonate reacts selectively with the single-stranded loops of cruciform structures. The irreversible inactivation of ribonuclease with diethylpyrocarbonate has been studied.

Packaging

5 g in glass bottle

25, 100 g in poly bottle