Application

Triton^™ X-100 has been used:Along with Span^® 80 and Tween^® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR techniqueAs a component of NX buffer which is used to pool and break down the microemulsions after PCR cyclingIn the blocking solution during immunocytochemistry procedureDuring deglycosylation procedureFor membrane solubilizationFor cell permeabilizationIn decellularization of porcine heart valve tissue

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

General description

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Legal Information

Span is a registered trademark of Croda International PLC

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

TWEEN is a registered trademark of Croda International PLC

Packaging

100, 500 mL in poly bottle

1 L in poly bottle