Application

Trizma^® hydrochloride along with Trizma-base is used to prepare buffer solutions of pH 7.35. It was used to prepare the triton extract. It may be used in the analysis of protease activity, isolated from the Bacillus stearothermophilus and Bacillus subtilis. It may be employed as buffer for the restriction fragment analysis of genomic DNA isolated from the enterococcal isolates and Escherichia coli.

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

General description

Trizma^® hydrochloride solution is a useful biological buffer.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 µm filtered.