Application

Trizma^® hydrochloride solution has been used: as a component of lysis buffer for lysing human embryonic kidney (HEK) 293 cellsin the 10X digoxigenin (DIG) deoxynucleoside triphosphate (dNTP) nick translation labeling buffer and in tris EDTA and sodium chloride (TEN) buffer in the preparation of tris, acetate, ethylenediaminetetraacetic acid (EDTA) (1x TAE) buffer and as a component of 10X gel loading dye

General description

Tris(hydroxymethyl)aminomethane (Tris) is a routine buffer in biological sciences. The pH buffering range is between 7.0 -9.0. It displays negligible binding towards metal in solutions. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. Its low ionic mobility finds usage in capillary electrochromatography as well.

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC