Application

Tris base has been used:To prepare Tris-HCl, which is used as a component of lysis buffer for protein characterizationIn the preparation of Tris-buffered saline Tween-20 (TBST) buffer for Western blottingIn the preparation of TBS (Tris-buffered saline) that is used for dialysis to neutralize a proteinAs a component of nuclei extraction buffer

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Packaging

2.5 kg in poly drum

1 kg in poly bottle