Application

L-α-Phosphatidylcholine is suitable for use:as a component of human erythroid massive amplification culture (HEMA^def) medium for clinical expansion of human erythroblastsin the chloride efflux assay to examine the chloride permeability of lipid vesicles conferred by soluble CLIC1in an assay to measure solubilized wax synthase activityas a component of buffer A used for the resuspension of the cholate-solubilized catalytic unit of adenylate cyclaseas a cryoprotectant for freezing ruminant spermas a substrate for the estimation of phospholipase activityto make liposomes to assess their efficiency of recruitment of ARF (ADP ribosylation factor) and AP-1 (adaptor protein)in the preparation of the isolated catalytic unit of soluble adenylate cyclase

Biochem/physiol Actions

Phosphatidylcholine is the major membrane phospholipid in eukaryotic cells, forming the structural component of these membranes. It also serves as a reservoir for several lipid messengers and a source for several bioactive lipids such as, lysophosphatidylcholine, phosphatidic acid, diacylglycerol, lysophosphatidylcholine, platelet activating factor, and arachidonic acid.

A major structural phospholipid in brain, comprising approx. 15% of total lipid; primarily localized to gray matter.

Packaging

25, 100, 500 g in poly bottle

1 kg in poly bottle

Preparation Note

Purified from P 5638