Application

D-Mannitol Colorimetric Assay Kit has been used to assess the intracellular mannitol content in samples.

General description

D-Mannitol is a common sugar alcohol (polyol) produced by many organisms including bacteria, fungi, and plants. D-Mannitol serves as a carbon storage compound, an osmoregulating agent, and antioxidant by protecting against reactive oxidative species (ROS). Mannitol is commonly used in food manufacturing as a reduced-calorie sweetener. The sugar alcohol L-arabitol also acts as a substrate for this assay; therefore, this kit can be used to measure L-arabitol.

Principle

The D-Mannitol Colorimetric Assay kit provides a simple and direct procedure for measuring D-mannitol in a variety of samples, including fruit and juices. D-Mannitol concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the D-mannitol present.

Suitability

Suitable for the detection of D-Mannitol/L-Arabitol in plant tissues, fungi, bacteria, food, juices, other beverages, etc.