AST Activity Assay Kit, sufficient for 100 colorimetric?�tests

Application

AST Activity Assay Kit has been used to determine the activity of aspartate aminotransferase in samples.

Features and Benefits

Compatible with high-throughput handling systems.

General description

Aspartate Aminotransferase (AST), formerly called serum glutamate-oxaloacetate transaminase (SGOT), is a pyridoxal phosphate (PLP)-dependent enzyme that catalyzes the conversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate. Similar to Alanine Aminotransferase (ALT), AST levels in blood are commonly used as a marker for liver function. However, AST has a broader tissue distribution than ALT and perturbations in AST levels can occur in response to diseases or injuries in multiple tissues including skeletal and heart.

Principle

The Aspartate Aminotransferase Activity Assay kit provides a simple and direct procedure for measuring AST activity in a variety of samples. In this kit, the transfer of an amino group from aspartate to α-ketoglutarate results in the generation of glutamate, resulting in the production of a colorimetric (450 nm) product proportional to the AST enzymatic activity present. One unit of AST is the amount of enzyme that will generate 1.0 µmole of glutamate per minute at pH 8.0 at 37 °C.

Suitability

Suitable for the measurement of aspartate aminotransferase (AST) activity in biological samples including tissue, cells and serum