Application

Hemin Assay Kit has been used to determine the level of free hemin (oxidized heme).

General description

Hemin, the oxidized version of heme, is an iron-containing prosthetic group for a diverse group of proteins. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Free hemin levels may be upregulated in various pathological conditions and can contribute to various inflammatory conditions including vascular disorders, renal failure, and immune-mediated disorders.

Principle

In this assay, Hemin concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm) product, proportional to the Hemin present.

Suitability

Suitable for use with cell and tissue culture supernatants, urine, plasma, serum, fecal material, media, and other biological fluids.