Application

Galactose and lactose assay kit has been used in the measurement of lactose/galactose concentration in milk/lactose-free milk and to estimate the saccharides concentrations measured with the biosensor.

General description

Lactose is a disaccharide containing one galactose and one glucose molecule. Lactose is the major sugar in the milk of most species, typically present between 2-8%. The enzyme lactase hydrolyzes lactose to its constituent monosaccharides. Galactose is a simple monosaccharide that serves as an energy source and as an essential component of glycolipids and glycoproteins. Galactose contributes to energy metabolism via its conversion to glucose by the enzymes that constitute the Leloir pathway. Defects in the genes encoding these proteins leads to the metabolic disorder galactosemia.

Principle

In this assay kit, galactose is oxidized by galactose oxidase resulting in a colorimetric (570 nm)/ fluorometric (λ_ex = 535 nm/λ_em = 587 nm) product, proportional to the galactose present. To detect lactose, lactose is hydrolyzed by lactase to generate free galactose, which is then measured (total galactose). The lactose level is equal to total galactose minus free galactose.

Suitability

Suitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.