HEPES, BioXtra, pH 5.0-6.5 (1 M in H2O), >=99.5% (titration)

Code: H7523-50G D2-231

Application

HEPES has been used:As a component of homogenisation buffer used in protein sample preparationTo neutralise PRP (platelet-rich plasma) solution as part of PRP pre...


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Application

HEPES has been used:As a component of homogenisation buffer used in protein sample preparationTo neutralise PRP (platelet-rich plasma) solution as part of PRP preparationAs a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparationAs a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

Packaging

50, 250 g in poly bottle

absorption≤0.035 at 280 in H2O at 1 M, ≤0.045 at 260 in H2O at 1 M
anion traceschloride (Cl-): ≤0.05%
application(s)diagnostic assay manufacturing
assay≥99.5% (titration)
cation tracesBi: ≤0.0005%, Fe: ≤0.0005%, Cr: ≤0.0005%, Ba: ≤0.0005%, Pb: ≤0.0005%, Al: ≤0.0005%, Ca: ≤0.001%, K: ≤0.005%, Cu: ≤0.0005%, Mo: ≤0.0005%, Mg: ≤0.0005%, Cd: ≤0.0005%, Ni: ≤0.0005%, Zn: ≤0.0005%, Mn: ≤0.0005%, Sr: ≤0.0005%, As: ≤0.0001%, Li: ≤0.0005%, Na: ≤0.005%, Co: ≤0.0005%
formpowder
ign. residue (900°C)≤0.1% (as SO4)
impuritiesInsoluble matter, passes filter test
InChI keyJKMHFZQWWAIEOD-UHFFFAOYSA-N
InChI1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
loss≤1.0% loss on drying, 110°C
pH5.0-6.5 (1 M in H2O)
pKa (25 °C)7.5
product lineBioXtra
Quality Level400
SMILES stringOCCN1CCN(CC1)CCS(O)(=O)=O
solubilityH2O: 1 M, clear, colorless
useful pH range6.8-8.2
Cas Number7365-45-9
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