Application
Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 µg/mL, specifically at 100 µg/mL for prokaryotes, 200 µg/mL for lower eukaryotes and 150-400 µg/mL for higher eukaryotes.
Biochem/physiol Actions
Mode of action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation. Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.
Caution
Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.
General description
Chemical structure: aminoglycoside
Preparation Note
This product is a solution that comes =60% (HPAE) and 45-60 mg/mL in H2O.
This product has met the following criteria: