Hygromycin B solution from Streptomyces hygroscopicus, >=60% (HPLC), 45-60 mg/mL in H2O

Code: H0654-1G D2-231

Application

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70...


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€957.00 1G
€1,177.11 inc. VAT

Application

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 µg/mL, specifically at 100 µg/mL for prokaryotes, 200 µg/mL for lower eukaryotes and 150-400 µg/mL for higher eukaryotes.

Biochem/physiol Actions

Mode of action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation. Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Caution

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.

General description

Chemical structure: aminoglycoside

Preparation Note

This product is a solution that comes =60% (HPAE) and 45-60 mg/mL in H2O.

antibiotic activity spectrumviruses
assay≥60% (HPLC)
biological sourceStreptomyces hygroscopicus
colorlight orange to dark orange, light yellow to dark yellow, brown-yellow
concentration45-60 mg/mL in H2O
InChI keyGRRNUXAQVGOGFE-XKIAHZFYSA-N
InChI1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
mode of actionprotein synthesis | interferes
Quality Level200
SMILES stringCN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
sterility0.22 µm filtered
storage temp.2-8°C
Code
Description
Unit Size
List Price
Qty
H0654-500MG
Unit:500MG
List Price: €533.00
Source:List Price
ADD
H0654-250MG
Unit:250MG
List Price: €311.00
Source:List Price
ADD
Cas Number31282-04-9
Hazard Class6.1
Un Number2810
Pack GroupI
This product has met the following criteria: