Trichloroacetic acid, BioUltra, >=99.5% (T)

Code: 91228-1KG D2-231

Application

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in mic...


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Application

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Trichloroacetic acid (TCA) has been used:to determine its effect on avian embryos during an exposure window encompassing cardiac specificationas a cell fixativeto terminate the O-acetylserine (thiol)lyase (OASTL) enzymatic assayfor the precipitation of acidic ribosomal proteins (RPs)

General description

Trichloroacetic acid (TCA) is derived from Trichloroethylene (TCE) metabolism. It is used as an acid decalcifying agent. TCA is used as a fixative for nuclear staining and protein precipitation.

Other Notes

Concentration of protein by quantitative precipitation; Cytochemical demonstration of DNA with a thiazine dye

λ0.5 M in H2O
anion traceschloride (Cl-): ≤30 mg/kg, nitrate (NO3-): ≤20 mg/kg, phosphate (PO43-): ≤10 mg/kg, sulfate (SO42-): ≤100 mg/kg
assay≥99.5% (T)
bp196 °C (lit.)
cation tracesBi: ≤5 mg/kg, Na: ≤50 mg/kg, Mo: ≤5 mg/kg, Al: ≤5 mg/kg, K: ≤50 mg/kg, As: ≤0.1 mg/kg, Li: ≤5 mg/kg, Cr: ≤5 mg/kg, Mn: ≤5 mg/kg, Sr: ≤5 mg/kg, Mg: ≤5 mg/kg, Ca: ≤10 mg/kg, Pb: ≤5 mg/kg, Cu: ≤5 mg/kg, Ba: ≤5 mg/kg, Fe: ≤5 mg/kg, Zn: ≤5 mg/kg, Ni: ≤5 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg
density1.62 g/mL at 25 °C (lit.)
formcrystals, flakes
ign. residue≤0.05%
impuritiesinsoluble matter, passes filter test
InChI keyYNJBWRMUSHSURL-UHFFFAOYSA-N
InChI1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)
mp54-58 °C (lit.)
pH﹤1.0 (25 °C, 0.5 M in H2O)
product lineBioUltra
Quality Level200
refractive indexn20/D 1.62 (lit.)
SMILES stringOC(=O)C(Cl)(Cl)Cl
solubilityH2O: 0.5 M at 20 °C, clear, colorless
UV absorptionλ: 280 nm Amax: 0.15, λ: 260 nm Amax: 1.60
vapor density﹤1 (vs air)
vapor pressure1 mmHg ( 51 °C)
Cas Number76-03-9
Hazard Class8
Un Number1839
Pack GroupII
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