Analysis Note

The Cytochrome C Reductase Assay Kit contains all the reagents required for a fast and convenient determination of NADPH-Cytochrome C Reductase activity in cell and tissue extracts and in purified microsomes. It has been tested on samples prepared from various species of mammalian tissues such as liver, kidney, brain, spleen, and heart muscle, as well as on lysates from cell lines such as HeLa, HepG2, and Jurkat. In addition, it has been tested on samples prepared from the yeast strains P. pastoris and S. cerevisiae.

Biochem/physiol Actions

Eukaroytic NADPH-cytochrome c reductase (NADPH-cytochrome P450 reductase, EC1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which are the cytochrome P450 family of enzymes, responsible for xenobiotic detoxification. NADPH-cytochrome c reductase is widely used as an endoplasmic reticulum marker and as one of the biomarkers for ecological pollution and dietary lipid uptake.

Features and Benefits

Detection of endoplasmic reticulum (ER) during isolation and subcellular fractionation by density gradient separation Colorimetric measurement of NADPH-Cytochrome C Reductase activity in various sources after intoxication by drugs or challenge by xenobiotics Analyze enrichment and function of ER