Analysis Note

Activity measured by ELISA using ganglioside GM₁ coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 µg of the cholera toxin B subunit-FITC conjugate per mL.

Application

Cholera Toxin B subunit-fluorescein isothiocyanate (FITC) conjugate has been used as a fluorescent tracer:to label distal cerebrospinal fluid-contacting neurons (dCSF-CNs) in lateral ventricles of ratto stain epididymalsperms of miceto stain peritoneal macrophages of mice

Biochem/physiol Actions

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Features and Benefits

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Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide