Anti-p-ULK1-Ser757, cl 5L18ZooMAb+Rb

Application

Peptide Inhibition Assay: Target band detection in lysate from serum starved A431 cells was prevented by preblocking of a representative lot (1:1,000 dilution) with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.Affinity Binding Assay: A representative lot of this antibody bound phosphorylated ULK1 (pSer757) peptide with a KD of 8.2 x 10-6 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Anti-phospho-ULK1-Ser757, clone 5L18 ZooMAb, Cat. No. ZRB1621, is a recombinant Rabbit monoclonal antibody that targets ULK1 phosphorylated on serine 757 and is tested in Affinity Binding Assay and Peptide Inhibition Assay, and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 757 from the C-terminal half of human ULK1.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 5L18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects ULK1 phosphorylated on serine 757.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Serine/threonine-protein kinase ULK1 (UniProt: O75385; also known as EC:2.7.11.1, Autophagy-related protein 1 homolog, ATG1, hATG1, Unc-51-like kinase 1) is encoded by the ULK1 (also known as KIAA0722) gene (Gene ID: 8408) in human. ULK1 is a ubiquitously expressed serine-threonine protein kinase that is involved in autophagy in response to starvation. During starvation it is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. In cells, ULK1 is constitutively in complex with at least three proteins: ATG13, FIP200 and ATG101. Interaction of ULK1 with ATG13 or FIP200 is reported to increase ULK1 kinase activity and stability. ULK1 undergoes autophosphorylation under nutrient-rich conditions and is dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, it is phosphorylated by MTOR/mTOR, which disrupts the interaction with AMPK and prevents activation of ULK1. In response to nutrient limitation it is phosphorylated and activated by AMPK that leads to activation of autophagy. It can also act as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulation of AMPK activity. Under nutrient sufficiency, high mTOR activity is reported to prevent ULK1 activation by phosphorylating ULK1 on serine 757 and disrupt its interaction with AMPK. The protein kinase domain of ULK1 is localized to amino acids 16-278. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zachari, M., and Ganley, IG. (2017). Essays Biochem. 61(6); 585-596; Kim, J., et al. (2011). Nat. Cell Biol. 13(2); 132-141).