Anti-Vimentin, clone 2I13 ZooMAb+ Rb

Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Vimentin in L6, U251, K562, and HEK293 cell lysates.Affinity Binding Assay: A representative lot of this antibody bound Vimentin peptide with a KD of 5.5 x 10-7 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Vimentin in human kidney tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Vimentin, clone 2I13 ZooMAb, Cat. No. ZRB1584, is a recombinant Rabbit monoclonal antibody that specifically targets Vimentin and is tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human Vimentin.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 2I13 is a rabbit recombinant monoclonal antibody that specifically detects Vimentin. It targets an epitope within 15 amino acids from the N-terminal half.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Vimentin (UniProt: P08670) is encoded by the VIM gene (Gene ID: 7431) in human. Vimentins are class-III intermediate filaments found in various non-muscle cells. It is the major subunit protein of the intermediate filaments of mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is usually expressed by cells undergoing cell-to-cell adhesion, EMT, and cytoskeleton remodeling. It is also involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin is also involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell. It has also been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin consists of a central -helical rod domain. The N-terminal part of the rod domain is a coil 1 motif that is organized into sequences of seven amino acid residues, where every first and fourth residue is hydrophobic. The C-terminal half of the rod domain has a coil 2 motif that shows a hydrophobic pattern. The vimentin structure shown to be dynamic in cells and can be rearranged to form filaments or the soluble tetrameric protein. The head and tail domains of vimentin contain over 35 phosphorylation sites and phosphorylation of its serine residues is reported to inhibit its subunit polymerization, thus promoting the disassembly of vimentin filaments and increasing its solubility. These phosphorylations also regulate protein-protein interactions and intracellular signaling. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Danielsson, F., et al. (2018). Cells. 7(10); 147; Lowery, J., et al. (2015). J. Biol. Chem. 290(28); 17145-17153).