Anti-p-Stat1-Tyr701,cl1F23ZooMAb� Rbmono

Application

Flow Cytometry Analysis: 1 µg from a representative lot detected phospho-Stat1-Tyr701 in HeLa cells treated with interferon a (2000 Units/mL; 20 min.)Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Stat1-Tyr701 in HeLa cells, serum starved and treated with interferon a (2000 Units/mL; 20 min.).Peptide Inhibition Assay: Target band detection in 10 µg of lysate from HeLa cells treated with interferon a was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.Affinity Binding Assay: A representative lot of this antibody bound phosphorylated Stat1-Tyr701 peptide with a KD of 7.9 x 10-6 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-p-Stat1-Tyr701, clone 1F23 ZooMAb, Cat. No. ZRB1520, is a recombinant Rabbit monoclonal antibody that targets p-Stat1 (Y701) and is used in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Peptide Inhibition Assay, and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phosphotyrosine 701 from the C-terminal region of human Signal transducer and activator of transcription 1 (Stat1).

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 1F23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Stat1 phosphorylated on Tyrosine 701. It targets an epitope within 12 amino acids surrounding phosphotyrosine 701 from the C-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Signal transducer and activator of transcription 1 (UniProt: P42224, also known as Transcription factor ISGF-3 components p91/p84) is encoded by the STAT1 gene (Gene ID: 6772) in human. STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling proteins. STAT1 is activated by many different ligands including interferons, interleukins, and growth factors. Following ligand binding to cell surface receptors, signaling via protein kinases leads to activation of JAK kinases and tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and enter the nucleus where it activates expression of multiple proinflammatory genes, including inducible nitric oxide synthase (iNOS) and cyclooxygenase. Loss of STAT1 activity can lead to early fatal infections and increased susceptibility to severe mycobacterial and viral infections. Phosphorylation of tyrosine 701 is required for STAT 1 dissociation from IFNGR1, homodimerization, and nuclear translocation. Tyrosine 701 phosphorylation impairment results in loss of STAT 1 functions. Phosphorylation on serine 727 by several kinases including MAPK14, ERK1/2 and CAMKII following on IFN-gamma stimulation, regulates STAT1 transcriptional activity. This phosphorylation also promotes sumoylation though increasing interaction with PIAS. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.