Anti-Bcl11A, clone 2C42 ZooMAb� Rb mono

Application

Anti-Bcl11A, clone 2C4 ZooMAb, Cat. No. ZRB1518, is a recombinant Rabbit monoclonal antibody that specifically targets Bcl11A and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: 1:1,000 dilution from a representative lot detected Bcl11A in Ramos and Jurkat cell lysates.Affinity Binding Assay: A representative lot of this antibody bound human recombinant Bcl11A with a KD of 2.2 x 10-8 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Bcl11A in human tonsil tissue sections.Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Bcl11A in HeLa cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

His-tagged recombinant fragment corresponding to 207 amino acids from the internal region of human B-cell lymphoma/leukemia 11A (Bcl11A).

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 2C4 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects B-cell lymphoma/leukemia 11A (Bcl11A). It targets an epitope within the C-terminal half.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

B-cell lymphoma/leukemia 11A (UniProt: Q9H165; also known as BCL-11A, B-cell CLL/lymphoma 11A, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 protein homolog, EVI-9, Zinc finger protein 856) is encoded by the BCL11A (also known as CTIP1, EVI9, KIAA1809, ZNF856) gene (Gene ID: 53335) in human. Bcl11A is a zinc-finger transcription factor that is involved in the generation of B-cell progenitor cells. It contains six C2H2 type zinc finger regions and recognizes TGACCA motif through zinc-finger domain. It is expressed at high levels in brain, spleen thymus, bone marrow and testis. Its expression has also been reported in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Its expression is tightly regulated during B-cell development. Several isoforms of Bcl11A have been reported. In adult erythroid cells, two isoforms are specifically expressed: Isoform 1, also known as Bcl11A-XL, is the more abundant form; Isoform 2, also known as Bcl11A-L, does not have the terminal zinc-finger region. Both these isoforms are predominantly located in the nucleus. Bcl11A associates with the BAF SWI/SNF chromatin remodeling complex. It is involved in hematopoiesis and brain development and is an essential factor in B-cell formation in fetal liver. Chromosomal aberrations involving BCL11A are associated with B-cell malignancies. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Liu, N., et al. (2018). Cell 173(2); 430-442).