Application

Anti-Integrin β3, clone EM00212 ZooMAb, Cat. No. ZRB1515, is a recombinant Rabbit monoclonal antibody that specifically targets Integrin β3 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Integrin β3 in SK-MEL-28, Hs294T, and A375 cell lysates.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Integrin β3 in human placenta and human melanoma tissue sections.Flow Cytometry Analysis: 1 βg from a representative lot detected Integrin β3 in one million HUVEC.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin β3 in HUVEC and SK-MEL-28 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

GST-tagged recombinant fragment corresponding to 41 amino acids from the C-terminal, cytoplasmic domain of human Integrin beta-3.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone EM00212 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Integrin β3. It targets an epitope within 41 amino acids from the C-terminal, cytoplasmic domain.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Integrin b-3 (UniProt: P05106; also known as Platelet membrane glycoprotein IIIa, GPIIIa, CD61) is encoded by the ITGB3 (also known as GP3A) gene (Gene: 3690) in human. Integrins are heterodimeric cell surface receptors composed of a variable alpha subunit and a conserved beta subunit. The exact combination of various alpha and beta subunits dictates the binding specificity of integrins to different ECM components. Integrins are not constitutively active. Activation of an integrin from low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. Integrin b-3 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to generate the mature form that contains a long extracellular domain (aa 27-718), a transmembrane domain (aa 719-741), and a cytoplasmic domain (aa 742-788). Integrin b-3 heterodimerizes with Integrin alpha-V or with Integrin-alpha IIb to serve as a receptor for fibronectin, vitronectin and other proteins. These heterodimers recognize the sequence RGD in a wide array of ligands. Mutations in ITGB3 gene are known to cause Glanzmann thrombasthenia that is characterized by mucocutaneous bleeding of mild-to-moderate severity. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.