Application

Anti-PIAS, clone 1O17 ZooMAb, Cat. No. ZRB1450, is a recombinant Rabbit monoclonal antibody that specifically targets PIAS and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PIAS in lysates from K562, L6, Sh-SY5Y, and NIH3T3 NE, and HeLa cell nuclear extracts. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PIAS in SH-SY5Y cells.Affinity Binding Assay: A representative lot of this antibody bound recombinant E3 Sumo protein fragment with a KD of 4.7 x 10-8 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected PIAS in human testis tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

GST/His-tagged recombinant fragment corresponding to 150 amino acids from the C-terminal region of human E3 SUMO-protein ligase PIAS1 protein.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 1O17 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects E3 SUMO-protein ligase PIAS1. It targets an epitope within the C-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

E3 SUMO-protein ligase PIAS1 (UniProt: O75925; also known as DEAD/H box-binding protein 1, E3 SUMO-protein transferase PIAS1, Gu-binding protein, GBP, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein) is encoded by the PIAS1 (also known as DDXBP1) gene (Gene ID: 8554) in human. PIAS is an E3-type small ubiquitin-like modifier (SUMO) ligase that is expressed in numerous tissues with highest levels found in the testis. It stabilizes the interaction between UBE2I and the substrate and also acts as a SUMO-tethering factor. PIAS plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. It is known to enhance the sumoylation of MTA1 and may also participate in its paralog-selective sumoylation. It also plays a role in adipogenesis by promoting the SUMOylation and degradation of CEBPB. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.