Anti-MICB, clone 2K9 ZooMAb� Rb mono

Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MICB in HeLa cell lysate.Flow Cytometry Analysis: 0.1 µg from a representative lot detected MICB in HeLa cells.Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected MICB in HeLa, A431, HepG2, HUVEC, and NIH 3T3 cell lines.Affinity Binding Assay: A representative lot of this antibody bound MICB peptide with a KD of 1.0 X 10-6 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-MICB, clone 2K9 ZooMAb, Cat No. ZRB1439, is a recombinant Rabbit monoclonal antibody that specifically targets MICB and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal, extracellular domain of human MHC class I polypeptide-related sequence B (MICB).

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in lyophilized from PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 2K9 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human MHC class I polypeptide-related sequence B (MICB). It targets an epitope within 20 amino acids from the N-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

MHC class I polypeptide-related sequence B (UniProt: Q29980; also known as MIC-B, MICB) is encoded by the MICB (also known as PERB11.2) gene (Gene ID: 4277) in human. MICB is a single-pass type I membrane protein that is widely expressed and acts as a stress-induced self-antigen. It is widely expressed with the exception of the central nervous system. It is also expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 23-309), a transmembrane domain (aa 310-330), and a cytoplasmic domain (aa 331-383). MICB can be induced by heat shock, oxidative stress, retinoic acid, interferon alpha, and 5-aza-2′-deoxycytidine. Its induction by interferon alpha is impaired in subjects with chronic hepatitis C virus infection. It is recognized by gamma-delta T cells and serves as ligands for the KLRK1/NKG2D receptor. Its binding to KLRK1 leads to cell lysis. Mutations in MICB gene have been linked to Rheumatoid arthritis that is characterized by inflammatory changes in the synovial membranes and articular structures, along with widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.