Anti-Angiopoietin-2,cl4L18 ZooMAb�RbMono

Application

Anti-Angiopoietin-2, clone 4L18 ZooMAb, Cat. No. ZRB1414, is a recombinant Rabbit monoclonal antibody that targets Angiopoietin-2 and is tested in Affinity Binding, Immunohistochemistry, Immunofluorescence, Western Blotting.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Angiopoietin-2 in post-fixed, frozen human placenta tissue sections.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Angiopoietin-2 in human placenta tissue sections.Affinity Binding Assay: Affinity Binding Assay: A representative lot of this antibody bound Angiopoietin-2 peptide with a KD of 4.6 x 10-7 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugate linear peptide corresponding to 23 amino acids from the N-terminal region of human Angiopoietin-2.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 4L18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Angiopoietin-2. It targets an epitope within 23 amino acids from the N-terminal region.

Storage and Stability

Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Angiopoietin-2 (UniProt: O15123; also known as ANG-2) is encoded by the ANGPT2 gene (Gene ID: 285) in human. Angiopoietins are a family of growth factors that include Angiopoietin 1 and 2 and the orthologs 3 (in mouse) and 4 (in human). ANG-2 is a secreted glycoprotein that is expressed predominantly by endothelial cells and some smooth muscle cells in a tightly regulated manner. High expression of ANG-2 has been observed in placenta. In quiescent mature vessels its expression is shown to be low; however, upon receipt of inflammatory signals its levels are significantly elevated. ANG-2 binds to TEK/TIE2 and competes for the Angiopoietin-1 (ANG-1) binding site and modulates ANG-1 signaling. It is also reported to induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANG-1. Both ANG-1 and ANG-2 have been implicated in tumor angiogenesis and ANG-2 is highly up-regulated in the vasculature of mouse and human neoplasms. It also acts as a chemoattractant and mediates the migration of a monocytes that express Tie2 receptor. ANG-2 contains a fibrinogen C-terminal domain (aa 275-495) that mediates its interaction with the TEK/TIE2 receptor. Three isoforms of ANG-2 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Scholz, A., et al. (2015). Ann. N.Y. Acad. Sci. 1347; 45-51; Felcht, M., et al. (2012). J. Clin. Invest. 122(6); 1991-2005).