Anti-Anillin, clone 2C6 ZooMAb� RbMono

Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Anillin in NIH/3T3 cell lysate.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Anillin in human testis tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Anillin in HUVEC and HeLa cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Anti-Anillin, clone 2C6 ZooMAb, Cat. No. ZRB1407, is a recombinant Rabbit monoclonal antibody that specifically targets Anillin and is tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

GST/His-tagged recombinant fragment corresponding to 150 amino acids from the N-terminal region of human Anillin.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.03 mg/mL after reconstitution at 25µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 2C6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Anillin. It targets an epitope within the N-terminal region.

Storage and Stability

Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Anillin (UniProt: Q9NQW6; also known as ANLN, Anillin acting binding protein) is encoded by the ANLN gene (Gene ID: 54443) in human. Anillin is a scaffold protein that links the actomyosin ring to membrane and RhoA. It is ubiquitously expressed with high levels found in the brain, placenta, and testis. It is also overexpressed in many tumor types including breast, colorectal, hepatic, pancreatic, and kidney tumors. In dividing cells, Anillin expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter the G1 phase. It is mainly found in the nucleus during interphase and colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis. Subsequently it concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody. Anillin is shown to be essential for cytokinesis and it maintains the structural integrity of the cleavage furrow. Anillin contains a conserved N-terminal actin and myosin binding domains, an Anillin homology domain and PH domain at the C terminus that is required for robust furrow localization and for its function. Anillin is reported to both enhance and limit contractility during cytokinesis through binding directly to active RhoA as well as several proteins that positively or negatively regulate Rho, and by crosslinking F-actin. Anillin is shown to be highly phosphorylated in mitosis and serves as a substrate for mitotic kinases. In human cells Anillin can be phosphorylated on 46 different residues. Phosphorylation at serine 635 is a key to mediate cytokinesis. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Piekny, AJ., and, Glotzer, M. (2008). Curr. Biol. 18(1); 30-36; Kim, H., et al. (2017). PLoS Genet 13(1); e1006511; Arnold, TR., et al., (2019). eLife.8; e39065).