Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MYO1C in HT29, HEK293, and NIH3T3 cell lysates.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MYO1C in human stomach and human tonsil tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MYO1C in HeLa, A431, HUVEC, NIH 3T3, and HT-29 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Anti-MYO1C, clone 4B19 ZooMAb, Cat. No. ZRB1372, , a recombinant Rabbit monoclonal antibody that targets Unconventional myosin-Ic (MYO1C) and is tested in Immunocytochemistry and Immunohistochemistry, and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to the first 16 amino acids from human Unconventional myosin-Ic, isoform 3.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 4B19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Unconventional myosin-Ic. It targets an epitope within 16 amino acids from the N-terminal region. May also display reactivity with isoform 1.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Unconventional myosin-Ic (UniProt: O00159; also known as Myosin I beta, MMI-beta, MMIb, MYO1C) is encoded by the MYO1C gene (Gene ID: 4641) in human. Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Myosins form regular bipolar thick filaments that together with actin thin filaments constitute the fundamental contractile unit of skeletal and cardiac muscle. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments. MYO1C, a single-headed class I myosin, localized to the cell surface. It is associated with cholesterol-enriched lipid rafts and enhances their recycling from intracellular compartments to the cell surface. It is involved in glucose transporter recycling in response to insulin by regulating movement of intracellular GLUT4-containing vesicles to the plasma membrane. It is reported to colocalize with RNA polymerase II in the nucleus and with RNA polymerase I in nucleoli. In the nucleolus, it is localized predominantly in the dense fibrillar component (DFC) and in granular component. The MYO1C gene produces three alternatively spliced isoforms that differ only in their N-terminal regions. Although they share identical motor domains, three calmodulin-binding IQ motifs containing a nuclear localization signal, and a membrane-binding tail domain, each isoform has different function and nuclear cytoplasm partitioning. These isoforms display both specific and overlapping nuclear and cytoplasmic functions and have different expression levels. Isoform 3 of MYO1C is involved in regulation of transcription and associates with transcriptional active ribosomal genes and cooperates with the WICH chromatin-remodeling complex to facilitate transcription. It is shown to be essential for the formation of the first phosphodiester bond during transcription initiation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zattelman L., et al. (2017). J Biol Chem. 292(43);17804-17818).