Application

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Tbr2 in NK-92 cell lysate.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Tbr2 in E16.5 mouse embryo tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Tbr2 in NTERA-2 an H9 cells.Affinity Binding Assay: A representative lot of this antibody bound Tbr2 peptide with a KD value of 4.6 x 10-8 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Anti-Tbr2, clone 3M12 ZooMAb, Cat. No. ZRB1368, a Rabbit monoclonal antibody that targets Tbr2 (Eomesodermin homolog) and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half of human TBR-2.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 3M12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects T-box brain proteinn 2 (Tbr2). It targets an epitope within 17 amino acids from the C-terminal half.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Eomesodermin homolog (UniProt: O95936; also known as T-box brain protein 2, T-brain-2, TBR-2) is encoded by the EOMES (also known as TBR2) gene (Gene ID: 8320) in human. T-box genes are a family of transcription factors have a highly conserved DNA binding domain named the T-domain. Tbr2 functions as a transcriptional activator that plays a crucial role during gastrulation, mesodermal specification, and limb patterning. It plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. It is considered as the major intrinsic determinants of the intermediate neural progenitors. It is reported to be transiently expressed in cortical subventricular zone progenitor cells. Tbr2 is also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Its DNA-binding region (T-Box) is localized to amino acids 276-456. Conditional inactivation of Tbr2 during early brain development is known to cause microcephaly and severe behavior deficits. Four isoforms of Tbr2 have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bulfone, A., et al. (1999). Mech. Dev. 84 (1-2); 133-138; Arnold, SJ., et al. (2008). Genes Dev. 22; 2479-2484; Sessa, A., et al. (2017). Cerebral Cortex. 27(6); 3378-3396).