Anti-Caspase-1 (p20), clone 2G4 ZooMAb�

Application

Anti-Caspase-1 (p20), clone 2G4 ZooMAb, Cat. No. ZRB1233, is a recombinant Rabbit monoclonal antibody that targets Caspase-1 proenzyme and p20 subunit and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Caspase-1 proenzyme and p20 subunit in rat spleen tissue lysate.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Caspase-1 (p20) in NIH 3T3 cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Caspase-1 (p20) in human small intestine tissue sections.Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the p20 subunit of human Caspase-1.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 2G4 is a ZooMAb rabbit recombinant monoclonal antibody that detects Caspase-1 proenzyme and the p20 subunit. It targets an epitope within 21 amino acids from the C-terminal region of p20 subunit.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Caspase-1 (UniProt: P29466; also known as EC:3.4.22.36, CASP-1, Interleukin-1 beta convertase, IL-1BC, Interleukin-1 beta-converting enzyme, ICE, IL-1 beta-converting enzyme, p45) is encoded by the CASP1 (also known as IL1BC, IL1BCE) gene (Gene ID: 834) inn human. Caspse-1 is a thiol protease that is synthesized as a single chain zymogen, which is further processed to create active caspase-1. The zymogen has two propeptides (aa 1-119 and 298-316), that are subsequently cleaved off to generate p20 (aa 120-297) and p10 (aa 317-404) subunits. The mature enzyme consists of two anti-parallel arranged heterodimers, each formed by a p20 and a p10 subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. It displays a strict requirement for an aspartic acid residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp. Caspase-1 cleaves IL-1 beta between an aspartic acid and alanine, releasing the mature cytokine which is involved in a variety of inflammatory processes. It is an important component of the inflammasome that during DNA virus infection controls antiviral immunity through cleavage of cGMP-AMP synthase (cGAS), rendering it inactive. Caspase-1 is expressed in larger amounts in spleen and lung, but is also detected in most other tissues, except for the brain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wang, Y., et al. (2017). Immunity. 46(3); 393-404).