Anti-Transferrin, clone 2B8 ZooMAb�

Application

Anti-Transferrin, clone 2B8 ZooMAb, Cat. No. ZRB1225, is a highly specific rabbit recombinant monoclonal antibody that targets Transferrin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Transferrin in human kidney tissue lysate.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Transferrin in NIH 3T3 and HepG2 cells.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Transferrin in human liver tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

GST/His-tagged recombinant fragment corresponding to 150 amino acids from the N-terminal half of the mature human Transferrin.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 2B8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human and murine Transferrin. It targets an epitope within the N-terminal region of mature transferrin.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Serotransferrin (UniProt: P02787; also known as Transferrin, Beta-1 metal-binding globulin, Siderophilin) is encoded by the TF (also known as PRO1400) gene (Gene ID: in human. Transferrin is a monomeric glycoprotein that is expressed mainly in the liver and is secreted in plasma. Its functions are mediated via binding to transferrin receptor. It serves as an iron binding and transport protein that can bind two Fe3+ ions in association with the binding of an anion, usually a bicarbonate. It transports iron from sites of absorption and heme degradation to those of storage and utilization. Transferrin is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature form. The mature form consists of alpha helices and beta sheets that form two domains. The N and C- terminal sequences are represented as globular N- and C- lobes that are connected with a short peptide. The carbohydrate moiety is attached to the C-lobe. Each lobe is further subdivided into two sub-domains- N1 and N2 for the N- lobe; and C1 and C2 for the C-lobe. Higher levels of transferrin are reported under conditions of anemia and during pregnancy. Mutations in TF gene are linked to Atransferrnemia that is characterized by abnormal synthesis of transferrin leading to iron overload and microcytic hypochromic anemia. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.