Anti-GST, clone 5B8 ZooMAb? 25 Y56

Application

Anti-GST, clone 5B8 ZooMAb, Cat. No. ZRB1223, is a recombinant Rabbit monoclonal antibody that specifically detects Glutathione S Transferase and has been tested for use in Dot Blot, ELISA, Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GST in HeLa cells transfeced with GST expression plasmid.ELISA Analysis: 0.5 µg/mL from a representative lot detected GST on plates coated with in GST, GST-TBP (Tata-Bindidng protein), and GST-beta-catenin.Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected various concentrations of GST-Tagged recombinant protein (portion of CD25 from amino acids 22-140), but not the His-Tagged recombinant protein.Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

Full-length recombinant E. Coli Glutathione S-transferase.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 5B8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Glutathione S Transferase.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Glutathione S-transferase (also known as GST) is encoded by the Gst gene in E. coli. Glutathione S transferases are a group of enzymes that are responsible for the detoxification of reactive chemical species through conjugation to reduced glutathione. They are ubiquitously distributed in nature, being found in organisms from microbes to higher mammals. They exist as membrane-bound as well as cytosolic enzymes with distinct catalytic and noncatalytic binding properties. They catalyze the conjugation of reduced glutathione (GSH) with compounds that contain an electrophilic center through the formation of a thioether bond between the sulfur atom of GSH and the substrate. The fundamental basis of their catalytic activity has been attributed to their capacity to lower the pKa of the sulfhydryl group of GSH from 9.0 in aqueous solution to about 6.5 when GSH is bound in the active site. In the case of certain substrates, GST can catalyze both the forward and reverse reactions, which can lead to increased toxicity rather than detoxication. GST has also been used as a tag for recombinant proteins where it can be fused with either the N- or the C-terminal of protein. Proteins produced using the GST fusion system have been used in numerous biological applications. It allows proteins to become more soluble. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Nebert, DW., and Vasiliou, V. (2004). Hum. Genomics. 1(6); 460-464).