Anti-Phospholamban,cl 4J16 ZooMAb�RbMono

Application

Anti-Phospholamban, clone 4J16 ZooMAb, Cat. No. ZRB1212, is a highly specific rabbit recombinant monoclonal antibody that targets Phospholamban and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Phospholamban in lysates from rat heart, and C2C12 and L6 cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from human Cardiac phospholamban.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 4J16 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Cardiac phospholamban. It targets an epitope within 23 amino acids from the N-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Cardiac phospholamban (UniProt: P26678; also known as PLB) is encoded by the PLN (also known as PLB) gene (Gene ID: 5350) in human. Phospholamban is a cardiac muscle-specific, 6 kDa, single-pass membrane protein that is shown to associate reversibly into pentamers. It modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. It modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of phospholamban. It is reported to tonically inhibit the SERCA pump by increasing its Km for Ca2+. Phosphorylation of phospholamban by protein kinase A at serine 16 is shown to relieve this inhibition, increasing the SR Ca-ATPase activity and lowering the cytoplasmic [Ca2+]. It can also undergo phosphorylation at threonine 17 by CaMK2 and in response to beta-adrenergic stimulation. Mutations in PLN gene have been linked to cardiomyopathies characterized by ventricular dilation and impaired systolic function that result in congestive heart failure and arrhythmia. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.