Application

Anti-Connexin 43, clone 5H23 ZooMAb, Cat. No. ZRB1179, is a highly specific recombinant rabbit monoclonal antibody that detects Connexin-43 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Connexin-43 in lysates from mouse and human brain and from human heart tissue.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Connexin-43 in human heart tissue sections.Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Connexin-43 in HUVEC ad A431 cells.Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Connexin-43.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 5H23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Connexin-43. It targets an epitope within 20 amino acids from the C-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Gap junction alpha-1 protein (UniProt: P17302; also known as Connexin-43, Cx43, Gap junction 43 kDa heart protein) is encoded by the GJA1 (also known as GJAL) (Gene ID: 2697) in human. Connexins assemble as hexamers (connexons) to form gap junctions that allow for the passage of ions and other small molecules between neighboring cells. Gap junctions play an important role in a variety of cellular processes including homeostasis, intercellular metabolic cooperation and synchronization, morphogenesis, cell differentiation, and growth control. Connexins usually have a short half-life, which is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. It has been detected in Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. It is phosphorylated at serine 368 by PRKCG and this phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity. Phosphorylation at serines 325, 328, and 330 by CK1 modulates gap junction assembly and phosphorylation at serine 368 by PRKCD can trigger its internalization into small vesicles leading to proteasome-mediated degradation. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.