Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Calnexin in Jurkat, NIH/3T3, and MCF7 cell lysates.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Calnexin in human tonsil and mouse testis tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Calnexin in HeLa, A431, HUVEC, NIH 3T3, Jurkat and MCF-7 cells.Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

Anti-Calnexin, clone 10N19 ZooMAb , Cat. No. ZRB1147, is a highly specific rabbit recombinant monoclonal antibody that targets Calnexin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin). and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Calnexin.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 10N19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Calnexin. It targets an epitope within 20 amino acids from the C-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Calnexin (UniProt: P27824; also known as IP90, Major histocompatibility complex class I antigen-binding protein p88, p90) is encoded by the CANX gene (Gene ID: 821) in human. Calnexin is a member of the calreticulin family of proteins. It is a well-conserved, single-pass type I membrane protein that serves as calcium binding, endoplasmic reticulum (ER) resident chaperone protein. It associates with newly synthesized, incompletely folded monomeric glycoproteins and promotes their correct folding prior to secretion. It is also reported to target misfolded proteins for degradation. It is anchored to the ER membrane by a transmembrane domain. Calnexin associates with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes and may, therefore, function as a signaling complex regulating thymocyte maturation. Calnexin is synthesized with a signal peptide of 20 amino acids, which is subsequently cleaved off to generate the mature form that has a lumenal domain (aa 21-481), a short transmembrane domain (aa 482-502) and a cytoplasmic domain (aa 503-592). Three isoforms of calnexin have been described that are generated by alternative splicing. Calnexin is shown to be phosphorylated at serine 564 by MAPK3/ERK1, which increases its association with ribosomes. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.