Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Lamin B1 in HUVEC and HeLa cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Lamin B1 in human kidney and mouse kidney tissue sections.Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

Anti-Lamin B1, clone 6K22 ZooMAb, Cat. No. ZRB1143, is a recombinant rabbit monoclonal antibody that specifically targets Lamin B1 and has been tested for use in Immunocytochemisry, Immunohistochemistry (Paraffin), and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

His-tagged recombinant fragment corresponding to 137 amino acids from the C-terminal region of human Lamin B1.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Lamin B1 (UniProt: P20700) is encoded by the LMNB1 (also known as LMN2, LNMB) gene (Gene ID: 4001) in human. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family proteins make up the matrix and are highly conserved. They are components of the nuclear lamina that provides a framework for the nuclear envelope. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Two types of lamins, A and B, exist in vertebrates. Type B lamins, B1 and B2, are present in all types of cells. Type A lamins are expressed only following gastrulation. Lamin A and C are the most common A-type lamins, they are both encoded by the LMNA gene and produced by alternative splicing. Lamin B1 is a homodimeric protein that is synthesized with a short propeptide (aa 584-586), which is removed in the mature form. The nuclear localization signal of Lamin B1 is localized to amino acids 415-420. Mutations in LMNB1 gene are reported to cause demyelinating Leukodystrophy that leads early autonomic abnormalities, pyramidal and cerebellar dysfunction and later symmetric demyelination of the CNS. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.