Anti-Integrin alpha V beta 6 Antibody; c

Application

Anti-Integrin alpha V beta 6, clone EM05201 ZooMAb , Cat. No. ZRB1104, is a recombinant Rabbit monoclonal antibody that specifically targets Integrin alphaV beta6 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Integrin alpha V beta 6 in human lung, rat lung, and rat teeth tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin alpha V beta 6 in HT-29 cells.Flow Cytometry Analysis: 0.1 µg from a representative lot detected Integrin alpha V beta 6 in HT-29 cells.Western Blot Analysis: A representative lot detected Integrin alpha V beta 6 in HT29 cell lysate.(Goodman, SL et al. (2012). Biol Open. 1(4):329-40).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

Recombinant transmembrane-truncated (DTM) extracellular domains of human integrins avb3, avb5, avb6, and avb8.Recombinant fragments corresponding to amino acids 31-987 of integrin alphaV and 24-706 of integrin beta6.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone EM05201 ZooMAb is a rabbit recombinant monoclonal antibody that specifically detects integrin alpha V beta 6.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Integrin alphaV beta6 (UniProt: P08648/P18564) is encoded by ITGA5/ITGB6 genes (Gene ID: 3678/3694) in human. Integrins are heterodimeric integral membrane proteins that control cell attachment. Fourteen different alpha chains are reported to associate with 8 beta chains to form 24 receptors. Integrins are differentially glycosylated, spliced, and activated in response to their cellular and extracellular environments. They function in cell surface adhesion and signaling. Integrin alphaV beta6 are single-pass type I heterodimeric membrane proteins that serve as a receptor for fibronectin and cytotactin. It is detected only in epithelial cell lines. It is not expressed in healthy adult epithelia but is shown to be upregulated during wound healing and in cancer. It recognizes the sequence RGD in its ligands. Internalization of integrin alphaV beta6 via clathrin-mediated endocytosis is reported to promote carcinoma cell invasion. Integrin alphaV beta6 is shown to mediate RGD-dependent release of TGF-beta-1 from regulatory latency-associated peptide (LAP) and plays a role in the activation of TGF-beta-1. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Weinacker, A., et al (1994). J. Biol. Chem. 269(9); 6940-6948; Goodman, SL., et al. (2012). Biol Open. 15;1(4):329-40).